pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB carpet white white - pGLO LB/amp 0 transparent N/a + pGLO LB/amp 110 white spots white + pGLO LB/amp/ara 640 yellow green fluorescent green

2.	What two new traits do your transformed bacteria have? The bacteria is resilient to ampicillin and it glows fluorescent green.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic. There were around 741 total bacteria colonies. we covered the bacteria with graph paper, measured the number of bacteria clumps in that area, then multiplied it by the number of spaces that the graph paper covered.
4.	What is the role of arabinose in the plates?
	Arabinose is used mainly as an inducer. Under regular temperatures and conditions the bacteria will not create fluorescent colors, but under the given conditions it will.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science. Many scientists are trying to see if it could someday cure cancer. Other scientists use it in plants and animals to see if they carry curtain plasmids. They can also figure out what different bacterias are living within organisms by putting the PGLO solution in it.
6.	Give an example of another application of genetic engineering.

Another application of genetic engineering are frost resistant plants, by giving the plant the PGLO plasmid and the frost resistant plasmid, we will know if it is frost resistant when we shine UV light on it and it glows fluorescent.